Free LAT Practice Questions
10 free, exam-style AALAS Laboratory Animal Technician (LAT) practice questions with answers and
explanations. No signup required. Work through them below, then take the
full free LAT practice test to study every exam domain.
Question 1
A newly hired technician is told that every warm-blooded animal in the facility is regulated under the Animal Welfare Act (AWA). When bred specifically for use in research, which of the following species is EXCLUDED from coverage under the AWA?
- Rabbits
- Guinea pigs
- Hamsters
- Mice
Show answer & explanation
Correct answer: D - Mice
Question 2
When using a gradual-fill carbon dioxide (CO₂) method to euthanize rodents, the current AVMA Guidelines recommend introducing CO₂ at a chamber displacement rate of:
- 30% to 70% of the chamber volume per minute
- 10% to 30% of the chamber volume per minute
- 5% to 10% of the chamber volume per minute
- 100% of the chamber volume per minute (rapid fill)
Show answer & explanation
Correct answer: A - 30% to 70% of the chamber volume per minute
Question 3
A drug is to be administered at a dose rate of 10 mg/kg to a rat weighing 250 g. What is the correct total dose?
- 0.25 mg
- 25 mg
- 2.5 mg
- 250 mg
Show answer & explanation
Correct answer: C - 2.5 mg
Question 4
In an institution's annual USDA report, a procedure that causes pain or distress that IS relieved through the appropriate use of anesthetics, analgesics, or tranquilizers is reported under which pain and distress category?
- Category B
- Category C
- Category D
- Category E
Show answer & explanation
Correct answer: C - Category D
Question 5
What is the key distinction between sterilization and disinfection?
- Sterilization removes visible soil, whereas disinfection does not
- Sterilization uses only chemical agents, whereas disinfection uses only heat
- Sterilization is used only on floors, whereas disinfection is used only on caging
- Sterilization destroys all microorganisms, including bacterial spores
Show answer & explanation
Correct answer: D - Sterilization destroys all microorganisms, including bacterial spores
Question 6
Guinea pigs lack the enzyme needed to synthesize a particular nutrient and will develop deficiency disease (e.g., scurvy) if it is not supplied in the diet. Which nutrient is it?
- Vitamin A
- Vitamin C
- Vitamin D
- Vitamin K
Show answer & explanation
Correct answer: B - Vitamin C
Question 7
According to the Guide for the Care and Use of Laboratory Animals, which range of fresh-air changes per hour is generally cited as an acceptable guideline for animal housing rooms?
- 10 to 15 air changes per hour
- 2 to 5 air changes per hour
- 20 to 25 air changes per hour
- 30 to 40 air changes per hour
Show answer & explanation
Correct answer: A - 10 to 15 air changes per hour
Question 8
A technician is bitten by a rhesus macaque while restraining it for an exam. Exposure to which zoonotic agent is the PRIMARY concern requiring immediate wound first aid and urgent medical evaluation?
- Lymphocytic choriomeningitis virus (LCMV)
- B virus (Macacine herpesvirus 1)
- Salmonella species
- Rabies virus
Show answer & explanation
Correct answer: B - B virus (Macacine herpesvirus 1)
Question 9
A technician makes a written error while recording an entry in a controlled-substance log. What is the correct way to correct the mistake?
- Use correction fluid to cover the error and write over it
- Erase the entry completely and write the correct information
- Completely black out the original entry so it cannot be read
- Draw a single line through it, then initial and date the change
Show answer & explanation
Correct answer: D - Draw a single line through it, then initial and date the change
Question 10
A room is used to house animals that have been intentionally infected with an infectious agent. To help contain that agent, the room should be maintained at which air pressure relative to the adjoining corridor?
- Positive air pressure relative to the corridor
- Negative air pressure relative to the corridor
- The same (neutral) pressure as the corridor
- Alternating positive and negative pressure
Show answer & explanation
Correct answer: B - Negative air pressure relative to the corridor